Merck Millipore HPC Total Count Sampler (25)

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Merck Millipore HPC Total Count Sampler
Merck Millipore samplers provide a means for simple, fast microbiological analyses of environmental waters, cooling tower waters, process waters, laboratory grade and electronics waters, dialysis water, and food and beverage products. Each sampler is constructed to combine both an intimate contact of a 0.45 µm membrane filter to a nutrient pad, and the incorporation of an air vent on the upper back portion of the paddle. This configuration allows for the draw-through of 1 mL of sample to affix microorganisms to the filter surface for subsequent culturing within its transparent plastic case. The filter is grid-marked to aid in counting the microbial colonies grown on its surface. Each sampler assembly is packaged in a sealed plastic envelope.

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Brand
BrandMerck Millipore
Description

Environmental Monitoring
Sample, incubate, count

Merck Millipore’s Samplers and Swab Test Kits simplify routine microbiological analysis of liquids and surfaces for bioburden levels of bacteria, yeast, or mould. This technique eliminates the cumbersome and expensive hardware and materials associated with traditional testing techniques.

Benefits
> Easy-to-Use: Just add a small sample of liquid to the Pre-sterilized Sampler or wipe a surface of interest with the Swab
> Ready-to-Use: Pre-measured dehydrated agar in the Sampler
> Stable at room temperature for storage and for incubation
> No instrument reader needed: Results achieved by visual counting of colonies
> Affordable: Low cost per test alleviates budget concerns

The Testing is as simple as
1. Sample: Obtain a sample. Samplers are filled to a top line. Swabs are wiped across a surface.
2. Incubate: Place in an incubator oven or incubate at room temperature. See incubation conditions below.
3. Count: Identify colonies of bacteria, yeast, and/or mould that are growing in your sample.
Results are available in as little as 22 – 72 hours using an incubator or in 7 days at room temperature depending on the test. Recoveries of microorganism levels are equivalent to pour plates, streak plates, and open funnel filtration in some cases.

Liquid Monitoring using Samplers
A Sampler consists of two parts:
> A plastic dip test handle with a 0.45 µm Millipore filter and an absorbent pad. This part contains the dehydrated nutrient medium for recovery of specific organisms.
> An outer plastic sheath. This piece is filled with the sample liquid of choice.
When the Sampler is immersed in a liquid, the absorbent pad rapidly absorbs 1 mL* into the filter membrane. Bacteria, yeast, or mould larger than the rated pore size are retained on the filter surface. The liquid hydrates the agar medium which provides nutrients to the organisms on the filter. These organisms will grow into defined colonies, which can be examined and counted. The number of bacteria, yeast, or mould colonies counted, can then be recorded and reported internally or externally.
* Samplers should only be used when counts >10 colonies/mL are anticipated. Samplers are not recommended for testing drinking water or when a 100 mL sample is required due to a low number of organisms.

Surface Monitoring using Swab Test Kits
Swab test kits combine a Sampler with a Swab. A Swab consists of two parts:
> A plastic cap connected to a polyester swab,
> An outer sheath containing pre-measured sterile phosphate buffer solution.
The Swab enables you to monitor surface cleanliness, test machine surfaces, and reach difficult areas where bacteria, yeast, or mould can grow.